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AUTHORS:
Robert
H. Demling, M.D. Leslie
DeSanti R.N.,
Dennis P. Orgill, M.D. Ph.D.
TRANSCYTE
A) OVERVIEW
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CHARACTERISTICS
- outer layer of
non-porous silicone (epidermal analog)
- inner membrane is a
layered product of human fibroblasts grown on a nylon mesh
(mainly collagen type I and fibronectin (dermal analog)
- fibroblasts removed and
product frozen -70° C to maintain activity of products
- adherence to clean wound
likely the result of collagen-fibrin and fibronectin-fibrin bond
- outer layer properties
likely that of silicone sheet
- must be stored at -70°
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PROPERTIES
- adherence
- water vapor control
- antibacterial
durability, flexibility
- antibiotic permeability
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No published
reports of the above properties for TransCyte use on a partial thickness wound
TRANSCYTE
PRODUCTION
Fibroblasts grown
on inner surface of Biobrane
TransCyte is stored
and sealed in a cassette with two pieces per cassette. Product is thawed just
prior to use.
B. ASSESSMENT OF PROPERTIES
OF TRANSCYTE SKIN SUBSTITUTE
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PROPERTIES
- adherence
- water vapor control
- * antibacterial
properties
- wound healing effects
- * antibiotic
permeability
- toxicity
* Not
reported in publications
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Adherence
Reported to be comparable with
allograft on excised wound. (Dermagraft-TC pivoted efficacy and safety study.
[ Purdue G, J Burn Care Rehab 1997: 18;13 ]
- water vapor control (evaporative
water loss)
Days Post Excision
Fluid accumulation beneath skin
substitute (0 to none to 5 heavy) [ Perdue G, JBCR ]
- wound healing (comparison with
topical antibiotics showed improved healing with TransCyte,
(Hanbrough 1997; JBCR) (Demling. 1999
Burns)
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Clinical
Use
- Mid dermal indeterminate
partial thickness burns with viable wound surface
- For excised burn wounds
with viable wound surface
- Donor sites (no
published data)
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Rules for
Use
- selection of appropriate
wounds (no adhesive to non-viable tissue)
- apply to clean wound bed
- apply gauze compression
dressing
- immobilize for 24 hrs,
larger over joints
- aspirate fluid, air
bubbles
- remove areas if with
persistent exudate
- remove when dry, opaque:
appearance similar to Biobrane when healed
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